Plant Image Collection

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abot92-9
Title
DNA fragmentation characteristic of programmed cell death (PCD) detected in NaCl
Author
Svetlana Shishkova Universidad Nacional Aut
Keywords
DNA fragmentation characteristic, programmed cell death, Stenocereus gummosus
Description
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DNA fragmentation characteristic of programmed cell death (PCD) was detected in NaCl-treated determinate primary roots of Stenocereus gummosus (Cactaceae) using the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay. Nuclei in the bottom left image, a longitudinal section of a root tip after PCD-inducing NaCl treatment, fluoresce blue after 4'-6-diamidino-2-phenylindole (DAPI) staining. Those nuclei undergoing DNA fragmentation fluoresce green in the same section (top left, fluorescein-isothiocyanate [FITC] staining). During root development, PCD was not involved in meristem exhaustion, but did occur in root-hair and root-cap cells. Both DNA and nuclear fragmentation were detected in a root-hair cell (right: top, FITC; middle, DAPI). Dead remnants (red-fluorescing nuclei, propidium-iodine stain, bottom right) of the root cap lie below a root that has terminated growth.
Publisher
Botanical Society of America
Additional data
copyright: Svetlana Shishkova, Joseph G. Dubrovsky, BSA
license: http://images.botany.org/index.html#license
Collection
Development and Morphogenesis Quick view
Botanical Name
Stenocereus gummosus
Link URL
http://www.amjbot.org/cgi/content/abstract/92/9/1590
Link Title
Developmental programmed cell death in primary roots of Sonoran Desert Cactaceae
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  • AJB Cover Image

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